ANSWERS FOR CPBA

OBJECTIVE

1. Transcribe CPBA. - COMPETITIVE PROTEIN BINDING ASSAY

2. Give a synonym for CPBA. - COMPETITIVE ASSAY

3 - 5. Enumerate the 3 most important
considerations for CPBA.
-RADIOLABELLED ANTIGEN, REAGENT ANTIBODY, UNKNOWN ANTIGEN

6 - 7. Enumerate two specific reagents for CPBA.
-RADIOLABELLED ANTIGEN, REAGENT ANTIBODY

8. What is the specific substance being tested in CPBA.
-UNKNOWN ANTIGEN

9. Give one specific test making use of the principle CPBA. - ELISA

10. What is a more reliable test? CPBA or EMIT? - EMIT, BECAUSE CPBA HAS INTERFERING SUBSTANCES

CASE ANALYSIS

In your own words, discuss the principle of CPBA through the use of the displayed picture on your right screen.

Cite one original example. Be creative.

I chose the picture Love Triangle of New Moon because it represent the CPBA. Edward is the unknown antigen, Bella is the reagent antibody and Jacob is the Radiolabeled antigen. As we all know, Jacob is competing for the love of Bella while Bella is deeply inlove with Edward!!

REFERENCES

Picture courtesy of WARNER BROTHERS