ANSWERS FOR CPBA

OBJECTIVE

1. Transcribe CPBA. - COMPETITIVE PROTEIN BINDING ASSAY

2. Give a synonym for CPBA. - COMPETITIVE ASSAY

3 - 5. Enumerate the 3 most important
considerations for CPBA.
-RADIOLABELLED ANTIGEN, REAGENT ANTIBODY, UNKNOWN ANTIGEN

6 - 7. Enumerate two specific reagents for CPBA.
-RADIOLABELLED ANTIGEN, REAGENT ANTIBODY

8. What is the specific substance being tested in CPBA.
-UNKNOWN ANTIGEN

9. Give one specific test making use of the principle CPBA. - ELISA

10. What is a more reliable test? CPBA or EMIT? - EMIT, BECAUSE CPBA HAS INTERFERING SUBSTANCES

CASE ANALYSIS

In your own words, discuss the principle of CPBA through the use of the displayed picture on your right screen.

Cite one original example. Be creative.

I chose the picture Love Triangle of New Moon because it represent the CPBA. Edward is the unknown antigen, Bella is the reagent antibody and Jacob is the Radiolabeled antigen. As we all know, Jacob is competing for the love of Bella while Bella is deeply inlove with Edward!!

REFERENCES

Picture courtesy of WARNER BROTHERS

QUESTIONS FOR CPBA

OBJECTIVE

1. Transcribe CPBA.

2. Give a synonym for CPBA.

3 - 5. Enumerate the 3 most important
considerations for CPBA.

6 - 7. Enumerate two specific reagents for CPBA.

8. What is the specific substance being tested in CPBA.

9. Give one specific test making use of the principle CPBA.

10. What is a more reliable test? CPBA or EMIT?

CASE ANALYSIS

In your own words, discuss the principle of CPBA through the use of the displayed picture on your right screen.

Cite one original example. Be creative.

REFERENCES

Picture courtesy of WARNER BROTHERS

CPBA

photo by rudyjansen

COMPETITIVE PROTEIN BINDING ASSAY

CPBA is also known as the competitive assay is a test used for quantitation of certain hormones like triiodothyronine. The principle of this test is generally revolving around the reagent antibody, labeled antigen and the unlabeled antigen which is the one were testing for. The labeled antigen and the unlabeled antigen compete for the binding site of reagent antibody.

There are many ways to measure them. The reagent antibody will be added to the labeled antigen then the unlabeled antigen is added. After the incubation, the free labeled antigen is separated and can be subtracted to the original number of the labeled antigen added or you can measure the bound antigen directly. The number of labeled antigen is pre-measured and should be fixed and small in amount and the number of the reagent antibody is also pre-measured and in small amount.

The more the binding of the reagent antibody and the labeled antigen, the less the number of binding of the labeled antigen thus making them indirectly proportional.

EXPLANATION OF THE PHOTO

I chose the picture Love Triangle because it represent the CPBA. the 2 girls are the unlabeled and the labeled antigen which are competing for the boy which represents as the reagent antibody.

REFERENCES

Clinical Chemistry Principles, Procedures, Correlations Fifth Edition by Bishop

http://www.flickr.com/photos/rudyjansen/2401083254/